Advances in network dynamics of in vitro neural systems


Oleg Vinogradov  | University of Tübingen, Germany
Paul Spitzner| Max Planck Institute for Dynamics and Self-Organization, Germany


In vitro cultures of neurons are one of the classical model systems in neuroscience that provide a great balance between complexity and experimental convenience. Due to this balance, in vitro cultures have supported the discovery of fundamental properties of neuronal networks, such as synaptic plasticity, E/I balance, and various homeostatic mechanisms. In recent years, major progress in experimental techniques and stem cells technologies has enabled greater control over the genetic and phenotypic heterogeneity of plated cells. Simultaneously, neuro-engineering and organoid technology allowed for unprecedented control over the topological structure of networks.

The goal of this workshop is to bring together experts in experiments, theory, and data analysis to explore insights into collective dynamics, network organization, and pathological pathways of complex neuropsychiatric disorders, which were enabled by recent advances in neural
models in vitro. We will also address ongoing experimental obstacles such as the variability of network dynamics between preparations, discuss recently discovered ways to enrich the complexity of network activity, and provide a perspective for future research that further closes the gap with in vivo systems.

Schedule (CEST)

Tuesday, Sept 13


Jordi Soriano | University of Barcelona, Spain
Rich dynamics and functional organization on topographically designed neuronal  networks in vitro


Paul Spitzner | Max Planck Institute for Dynamics and Self-Organization, Germany
Modular architecture facilitates noise-driven breakdown of synchrony in neuronal networks


Elisha Moses | Weizmann Institute of Science, Isreal
Overview of stimulation, connectivity and structure in cultured neural networks


Michela Chiappalone | University of Genova, Italy
Closed-loop neuroengineering: from in vitro to in vivo studies


Barbara Genocchi | Tampere University, Finland
New analysis methods to better depict astrocytes effects in neuron-astrocyte networks cultured on MEAs


30 min break


Samora Okujeni | University of Freiburg, Germany
Structural modularity promotes mesoscale criticality in neuronal networks in vitro


Martina Brofiga | University of Genoa, Italy
Brain-on-a-chip models to investigate the role of modularity, heterogeneity, and three-dimensionality on in vitro neuronal networks



End of first day

Wednesday, Sept 14


Oleg Vinogradov | University of Tübingen, Germany
Dynamical principles of network bursting in vitro


Pascal Monceau | Paris Diderot University, France
Modelisation of calcium driven synchronization in hippocampal neuronal cultures


Shani Stern | University of Haifa, Israel
Reduced Synaptic Activity and Dysregulated Extracellular Matrix Pathways in Midbrain Neurons from Parkinson’s Disease Patients


Michael Ziller | University of Münster, Germany
Polygenically driven alternative polyadenylation mediates synaptic impairment and E/I balance deficits in iPSC derived neuronal microcircuits from schizophrenia patients


30 min break


Richard Gao | University of Tübingen, Germany
Simulation-based inference for discovering mechanistic models of network dynamics in brain organoids


Liset M de la Prida | Instituto Cajal, Spain
Understanding brain oscillatory regimes in vitro versus in vivo


Panel discussion